CROMATEST ALKALINE PHOSPHATASE BIOCHEMISTRY REAGENT

CROMATEST ALKALINE PHOSPHATASE BIOCHEMISTRY REAGENT Overview

The CROMATEST ALKALINE PHOSPHATASE BIOCHEMISTRY REAGENT is an in vitro diagnostic solution designed for the quantitative kinetic determination of Alkaline Phosphatase (ALP) activity in human serum and heparinized plasma. Utilizing a highly specific 4-Nitrophenyl Phosphate (4-NPP) substrate method, this reagent is essential for the clinical diagnosis of hepatobiliary diseases and bone disorders. It offers laboratories in Bangladesh a reliable, cost-effective solution for monitoring liver function and bone metabolism with high analytical precision.

CROMATEST ALKALINE PHOSPHATASE BIOCHEMISTRY REAGENT Key Features

• High-Precision Kinetic Methodology: Monitors the reaction at 405 nm to accurately measure the rate of 4-nitrophenol formation, ensuring results are directly proportional to ALP activity in the sample.
• Optimized Reagent Composition: Features a stable DEA buffer (pH 10.2) and Magnesium Chloride activator, providing an ideal environment for enzymatic hydrolysis.
• Enhanced Stability & Storage: Reagents remain stable until the expiry date when stored at 2-8°C. The prepared working reagent offers flexibility with 5 days stability at room temperature (20-25°C).
• Broad Clinical Utility: Critical for differentiating between bone diseases (e.g., Paget’s disease, rickets) and hepatobiliary conditions (e.g., biliary obstruction, drug-induced cholestasis).
• Interference Resistant: Formulated to minimize interference from bilirubin (up to 20 mg/dL) and triglycerides (up to 10 g/L), reducing the rate of false positives.

Technical Specifications

• Principle: Kinetic enzymatic hydrolysis of 4-Nitrophenyl Phosphate (4-NPP).
• Wavelength: 405 nm.
• Reagent Composition:
  • R1 (Buffer): DEA buffer 1.25 mol/L (pH 10.2), Magnesium chloride 0.6 mmol/L, Biocides.
  • R2 (Substrate): 4-NPP 50 mmol/L, Biocides.
• Working Reagent Ratio: R1 : R2 = 4 mL : 1 mL.
• Sample Type: Serum or Heparinized Plasma (Hemolysis-free). Note: Do not use EDTA, Oxalate, or Citrate anticoagulants.
• Linear Range: Up to 800 U/L.
• Calculation Formula: U/L = ΔA/min × 2764.
• Storage Requirements: 2-8°C (Reagents), 2-8°C or 20-25°C (Working Reagent).
• Instrumentation Required: Photometer/Spectrophotometer with thermostatted cell (25/30/37°C), stopwatch, cuvettes (1 cm), and precise pipettes.