Urea Berthelot Biochemistry Reagent – 200Test

Urea Berthelot Biochemistry Reagent – 200Test Overview

The Urea Berthelot Biochemistry Reagent is a premium diagnostic solution designed for the quantitative in vitro determination of urea concentration in human serum, plasma, and urine samples. Utilizing the classic Berthelot reaction principle, this reagent system detects the formation of a blue chromophore proportional to urea levels, providing critical insights into kidney function and protein metabolism. It is an essential tool for clinical laboratories in Bangladesh performing renal function panels.

Urea Berthelot Biochemistry Reagent – 200Test Key Features

• High-Precision Principle: Based on the hydrolysis of urea by urease, reacting with alkaline hypochlorite and sodium salicylate to yield a measurable blue chromophore.
• Comprehensive Kit: Includes R1 (Enzyme), R2 (Buffered Chromogen), R3 (Alkaline Hypochlorite), and a Urea Standard for accurate calibration.
• Optimized Stability: Working reagents remain stable for up to 4 weeks at 2-8°C, ensuring minimal waste and reliable batch testing.
• Proven Accuracy: Demonstrates excellent correlation with commercial methods (r = 0.999) and low CV% for both within-run and between-run precision.
• Interference Resistance: Formulated to resist interference from bilirubin and triglycerides, ensuring clean results even with complex samples.

Technical Specifications

• Test Capacity: 200 Tests per kit.
• Reagent Composition:
  • R1: Urease > 500 U/mL + Stabilizers
  • R2: Phosphate buffer (20 mmol/L), EDTA, Sodium Salicylate (60 mmol/L), Sodium Nitroprusside (3.4 mmol/L)
  • R3: Sodium Hypochlorite (10 mmol/L), NaOH (150 mmol/L)
  • CAL: Urea Standard 50 mg/dL (8.3 mmol/L)
• Methodology: Berthelot Method (Endpoint).
• Required Instrumentation: Photometer or Colorimeter capable of measuring absorbance at 600 nm (± 10 nm) and a constant temperature incubator set at 37°C.
• Sample Type: Serum, Heparinized Plasma (free of hemolysis), and Urine.
• Storage Conditions: Store at 2°C to 8°C (Refrigerated).
• Linearity: Up to 300 mg/dL (Samples exceeding this must be diluted 1:5).
• Performance Metrics:
  • Within-run Precision (CV%): < 1.33%
  • Between-run Precision (CV%): < 3.9%
  • Sensitivity: 1 mg urea produces net absorbance of ~0.002.