Cromatest Total Bilirubin Biochemistry Reagent – 1000 Test

Cromatest Total Bilirubin Biochemistry Reagent – 1000 Test Overview

The Cromatest Total Bilirubin Biochemistry Reagent is a high-precision diagnostic solution designed for the quantitative in vitro determination of total bilirubin in human serum and plasma. Utilizing the established azobilirubin diazo method, this reagent converts bilirubin into a colored azobilirubin complex, which is measured photometrically at 540 nm. It is an essential tool for diagnosing hyperbilirubinemia, monitoring liver function, and detecting hemolytic disorders in clinical laboratories across Bangladesh. This kit provides a cost-effective solution for high-volume testing, offering a total yield of 1000 tests.

Cromatest Total Bilirubin Biochemistry Reagent – 1000 Test Key Features

• High-Performance Diazo Methodology: Accurately measures total bilirubin by reacting direct bilirubin and displacing albumin-bound (indirect) bilirubin using a specialized accelerator.
• Extensive Test Capacity: Bulk packaging provides 1000 tests, optimizing workflow efficiency and reducing procurement costs for busy hospital and diagnostic labs.
• Superior Analytical Precision: Demonstrates excellent precision with a CV% as low as 0.4% and linearity up to 20 mg/dL, ensuring reliable and reproducible results.
• Long Working Stability: Once reconstituted, the working reagent remains stable for 8 days when refrigerated at 2-8°C, minimizing waste.
• Traceable Quality Control: Includes a bilirubin calibrator traceable to SRM 916a, ensuring accuracy independent of the instrument used.
• Automated Analyzer Compatibility: Formulated for easy adaptation on most standard photometers and automated biochemistry analyzers.

Technical Specifications

• Principle: Photometric determination via diazotization of sulfanilic acid (Azobilirubin method).
• Reagent Composition:
  • RT (Reagent 1): Sulfanilic acid 29 mmol/L, Hydrochloric acid 0.24 mol/L, Duposol® 3% (w/v).
  • RN (Reagent 2): Sodium nitrite 11.6 mmol/L.
• Wavelength: 540 ± 20 nm.
• Incubation Temperature: 37°C (standard).
• Sample Type: Fresh hemolysis-free serum or EDTA/Heparinized plasma.
• Storage Conditions: Store refrigerated at 2-8°C. Protect from light.
• Linearity: Up to 20 mg/dL (342 µmol/L).
• Precision (CV%): Within-run 0.4% – 1.1%; Between-run 0.4% – 2.8%.
• Interference: Lipemic samples may interfere; hemoglobin up to 1g/dL has negligible effect.