OXOID Tryptone Soya Broth (TSB)

OXOID Tryptone Soya Broth (TSB) – Premium Liquid Culture Medium for Microbiological Growth

OXOID Tryptone Soya Broth (TSB) is a high-quality, general-purpose liquid culture medium designed for the cultivation of a wide variety of microorganisms. Produced by Oxoid, this broth is widely trusted in clinical, pharmaceutical, food, and research laboratories for its consistent performance and rich nutrient composition.

 What is Tryptone Soya Broth (TSB)?

Tryptone Soya Broth is a non-selective, nutrient-rich liquid medium made from enzymatic digests of casein and soybean meal. It supports the growth of both fastidious and non-fastidious organisms, making it ideal for routine microbial culture and enrichment.

 Key Features

• General Purpose Broth – Suitable for a broad spectrum of bacteria and fungi
• Highly Nutritive Formula – Promotes rapid and luxuriant growth
• Excellent for Enrichment – Ideal for low-level microbial detection
• Consistent Quality – Reliable batch-to-batch performance
• Widely Accepted Standard – Used in global laboratory protocols

 Composition (Typical per Liter)

• Tryptone (Pancreatic digest of casein): 17.0 g
• Soya peptone (Papaic digest of soybean meal): 3.0 g
• Sodium chloride: 5.0 g
• Dipotassium phosphate: 2.5 g
• Glucose: 2.5 g
• Final pH: 7.3 ± 0.2 at 25°C

 Applications

• Sterility Testing – Widely used in pharmaceutical quality control
• Microbial Enrichment – Recovery of stressed or low-count organisms
• Culture Preparation – Growth of inoculum for further testing
• Food & Beverage Testing – Detection of microbial contamination
• Environmental Monitoring – Water and surface sample testing

 Directions for Use

1. Dissolve the required quantity of TSB powder in distilled water.
2. Mix thoroughly and heat gently if necessary to dissolve.
3. Sterilize by autoclaving at 121°C for 15 minutes.
4. Cool before inoculation.
5. Incubate under appropriate conditions depending on the organism.

 Storage & Shelf Life

• Store in a cool, dry place (10–30°C)
• Protect from moisture and direct sunlight
• Keep the container tightly sealed

 Precautions

• For laboratory use only
• Maintain aseptic conditions during preparation and handling
• Do not use if the powder shows signs of contamination or moisture

 Why Choose OXOID TSB?

Backed by the global reputation of Oxoid, Tryptone Soya Broth delivers dependable performance, making it a preferred choice for laboratories that demand accuracy and consistency in microbiological testing.