OXOID Nutrient Broth

OXOID Nutrient Broth – General Purpose Liquid Culture Medium

OXOID Nutrient Broth is a high-quality, general-purpose liquid culture medium designed for the growth of a wide variety of non-fastidious microorganisms. Produced by Oxoid, this broth is widely used in clinical, research, food, and pharmaceutical laboratories for routine microbial cultivation and enrichment.

 What is Nutrient Broth?

Nutrient Broth is a non-selective, nutrient-rich liquid medium that provides essential nutrients, vitamins, and salts to support the growth of bacteria and fungi. It is ideal for the propagation, enrichment, and maintenance of microbial cultures.

 Key Features

• General Purpose Broth – Supports growth of a broad range of microorganisms
• Highly Nutritive – Contains peptones, beef extract, and salts for optimal microbial growth
• Consistent Performance – Reliable batch-to-batch quality
• Versatile Applications – Used in routine microbiology, quality control, and environmental testing

 Composition (Typical per Liter)

• Peptone: 5.0 g
• Beef extract: 3.0 g
• Sodium chloride: 5.0 g
• Final pH: 7.0 ± 0.2 at 25°C

 Applications

• Routine Microbial Cultivation – Growth and maintenance of bacterial and fungal cultures
• Enrichment Medium – Recovery of stressed or low-count organisms
• Microbial Enumeration – Total viable counts (TVC) in samples
• Environmental Testing – Air, water, and surface contamination monitoring
• Pharmaceutical & Food QC – Sterility and microbial limit testing

 Directions for Use

1. Dissolve the required amount of Nutrient Broth powder in distilled water.
2. Mix thoroughly and heat gently if needed to dissolve completely.
3. Sterilize by autoclaving at 121°C for 15 minutes.
4. Cool before inoculation.
5. Incubate under appropriate conditions for the organism.

 Storage & Shelf Life

• Store in a cool, dry place (10–30°C)
• Protect from moisture and direct sunlight
• Keep container tightly closed

 Precautions

• For laboratory use only
• Maintain aseptic techniques during preparation and handling
• Avoid using contaminated or wet powder

Why Choose OXOID Nutrient Broth?

With the global reputation of Oxoid, Nutrient Broth provides consistent microbial growth, reliable results, and ease of use, making it an essential medium in microbiology laboratories worldwide.