Mast Mueller Hinton Agar 500gm

Mast Mueller Hinton Agar 500g – Dehydrated Microbiology Culture Media

Mast Mueller Hinton Agar (MHA) 500g is a premium dehydrated culture medium designed for Antimicrobial Susceptibility Testing (AST). Widely recognized as the standard medium for the Kirby-Bauer disk diffusion method, it is used in clinical, diagnostic, and research laboratories to evaluate the effectiveness of antibiotics against bacterial pathogens.

Manufactured to meet international standards, Mast MHA ensures consistent and reproducible results for reliable laboratory testing.

Intended Use

Recommended for determining the susceptibility of microorganisms to antimicrobial agents isolated from clinical samples. Ideal for routine AST procedures following CLSI guidelines.

Key Features

• Pack Size: 500g dehydrated powder

• Standard medium for AST & Kirby-Bauer method

• pH 7.3 ± 0.1 at 25°C

• High-quality, consistent batch performance

• Supports growth of most non-fastidious bacteria

• Easy to prepare and sterilize (autoclave at 121°C for 15 min)

• Suitable for clinical and research laboratories

General Composition & Function

• Beef Extract: Provides nitrogen, vitamins, and essential nutrients.

• Casein Hydrolysate: Source of amino acids and peptides.

• Starch: Absorbs bacterial toxins, ensuring accurate AST results.

• Agar: Solidifying agent for plates.

• Distilled Water: Used to dissolve all ingredients.

Applications

✔ Antimicrobial Susceptibility Testing (AST)
✔ Kirby-Bauer disk diffusion method
✔ Routine bacterial culture of non-fastidious organisms
✔ Clinical and diagnostic laboratories
✔ Pharmaceutical and research testing

Advantages

• Standardized and globally accepted medium for AST

• Ensures reproducible and reliable antibiotic susceptibility results

• Controlled mineral levels prevent interference with antibiotic diffusion

• Easy preparation for consistent laboratory use

Precautions

• Not suitable for fastidious organisms (e.g., Neisseria, Haemophilus)

• Maintain agar depth at 4 mm for accurate zone measurements

• Follow standard sterilization and preparation protocols

Mast Mueller Hinton Agar 500g is an essential culture medium for laboratories to accurately determine bacterial resistance and guide effective antibiotic therapy.