Colistin – 10 μg Antibiotic Disc – Bioanalyse

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Colistin – 10 μg Antibiotic Disc – Bioanalyse Overview

The Colistin – 10 μg Antibiotic Disc – Bioanalyse is a critical diagnostic tool for antimicrobial susceptibility testing (AST). This precisely manufactured paper disc, impregnated with 10 micrograms of Colistin, a potent polymyxin antibiotic, is essential for determining bacterial sensitivity to this last-resort treatment. It is specifically designed for use in the widely adopted disc diffusion method, providing laboratories with a reliable, cost-effective, and straightforward approach to identifying bacterial resistance patterns, particularly against challenging multidrug-resistant organisms like carbapenem-resistant Enterobacteriaceae (CRE) and Pseudomonas aeruginosa.

Colistin – 10 μg Antibiotic Disc – Bioanalyse Key Features

• Standardized Potency: Each disc contains a precise 10 μg concentration of Colistin, ensuring consistent and reproducible test results.
• Ease of Use: Designed for the standard disc diffusion method, making it simple to implement in any clinical or research laboratory setting.
• Reliable Performance: Contributes to accurate identification of bacterial susceptibility or resistance, aiding in effective treatment decisions.
• Cost-Effective Diagnosis: Offers an economical solution for routine antimicrobial susceptibility testing compared to more complex methods.
• High-Quality Impregnation: Ensures even distribution of the antibiotic for predictable diffusion and clear zone of inhibition formation.
• Trusted Brand: Manufactured by Bioanalyse, a reputable name in laboratory diagnostics, assuring quality and dependability.

Technical Specifications

• Product Name: Colistin – 10 μg Antibiotic Disc
• Manufacturer: Bioanalyse
• Antibiotic: Colistin
• Concentration: 10 μg per disc
• Testing Method: Disc Diffusion (Kirby-Bauer)
• Application: Antimicrobial Susceptibility Testing (AST)
• Material: High-quality, sterile absorbent paper
• Packaging: Provided in a format ensuring sterility and stability prior to use.
• Storage: Recommended to be stored under specified conditions to maintain efficacy (refer to manufacturer’s instructions for optimal temperature and humidity).

How to Use and Interpret Results

The Colistin – 10 μg Antibiotic Disc – Bioanalyse is used within the established disc diffusion protocol. A standardized bacterial suspension is inoculated onto an agar plate, followed by the placement of the Colistin disc. After incubation (typically 16-24 hours at 37°C), the diameter of the zone of inhibition around the disc is measured. Interpretation is based on established clinical breakpoints:

• Susceptible: Zone diameter ≥ 10 mm
• Intermediate: Zone diameter 6-9 mm
• Resistant: Zone diameter < 6 mm

Important Considerations: While the disc diffusion method is a valuable screening tool, it is considered semi-quantitative. For definitive or more precise results, methods like broth microdilution or agar dilution may be required. This test is crucial for guiding therapy against multidrug-resistant bacteria, but clinicians must also consider the potential nephrotoxicity of Colistin and utilize it judiciously as a last resort.

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