Trimethoprim/Sulphamethoxae – 25 μg Antibiotic Disc – Bioanalyse

Trimethoprim/Sulphamethoxae – 25 μg Antibiotic Disc – Bioanalyse Overview

The Trimethoprim/Sulphamethoxae – 25 μg Antibiotic Disc – Bioanalyse is a critical diagnostic tool for microbiology laboratories. This sterile paper disc, impregnated with a precise 25 microgram combination of Trimethoprim and Sulfamethoxazole, is essential for performing the disc diffusion method of antimicrobial susceptibility testing. It enables healthcare professionals to accurately determine the sensitivity of bacterial isolates to this broad-spectrum antibiotic, guiding effective treatment strategies for a wide range of infections. By evaluating the zone of inhibition, clinicians can confidently select appropriate antimicrobial therapy, ensuring optimal patient outcomes.

Trimethoprim/Sulphamethoxae – 25 μg Antibiotic Disc – Bioanalyse Key Features

• Accurate Susceptibility Testing: Facilitates reliable determination of bacterial susceptibility to Trimethoprim/Sulphamethoxazole.
• Broad-Spectrum Efficacy: Impregnated with a combination antibiotic effective against both Gram-positive and Gram-negative bacteria.
• Standardized Impregnation: Each disc contains a precise 25 μg concentration for consistent and reproducible results.
• Ease of Use: Designed for straightforward application in standard disc diffusion protocols.
• High-Quality Materials: Manufactured with premium filter paper for optimal diffusion characteristics.
• Trusted Brand: Produced by Bioanalyse, a reputable name in diagnostic reagents.

Technical Specifications

• Product: Antibiotic Disc
• Antibiotic Combination: Trimethoprim/Sulphamethoxazole
• Impregnation Strength: 25 μg per disc
• Testing Method: Disc Diffusion (Kirby-Bauer)
• Manufacturer: Bioanalyse
• Application: Antimicrobial Susceptibility Testing (AST)
• Disc Material: High-quality absorbent paper
• Intended Use: In vitro diagnostic use

Understanding Results: Zone of Inhibition Interpretation

Interpreting the zone of inhibition is crucial for accurate susceptibility assessment. Please refer to the following standard guidelines:

• Susceptible: Zone of inhibition ≥ 16 mm
• Intermediate: Zone of inhibition 11-15 mm
• Resistant: Zone of inhibition < 11 mm

Note: The disc diffusion method is a semi-quantitative technique. For definitive results or to detect subtle resistance mechanisms, consider complementary methods such as broth microdilution or agar dilution.

Important Considerations and Limitations

• The accuracy of the disc diffusion method can be influenced by factors such as agar type, incubation temperature, and inoculum density. Adherence to standardized laboratory procedures is essential.
• Certain bacterial strains, including some resistant strains of Escherichia coli, may not exhibit a detectable zone of inhibition despite resistance to Trimethoprim/Sulphamethoxazole.

Safety Information

Trimethoprim/Sulphamethoxazole is a potent antibiotic. While these discs are for in vitro diagnostic use, awareness of potential side effects associated with the antibiotic itself is important. These can include nausea, vomiting, diarrhea, abdominal pain, headache, dizziness, rash, liver damage, and blood disorders. Always consult with a healthcare professional regarding any concerns. Inform your doctor or pharmacist about all medications you are taking, as interactions may occur.