Methicillin – 5 μg Antibiotic Disc – Bioanalyse

Methicillin – 5 μg Antibiotic Disc – Bioanalyse Overview

The Methicillin – 5 μg Antibiotic Disc – Bioanalyse is a critical diagnostic tool for microbiology laboratories, specifically designed for the disc diffusion method of antimicrobial susceptibility testing. Each precisely manufactured paper disc is impregnated with 5 micrograms of Methicillin, a key penicillin-class antibiotic. This product enables healthcare professionals to accurately determine the susceptibility of bacterial isolates, particularly in identifying resistance patterns that are crucial for effective treatment strategies. It is an essential component in the fight against antibiotic resistance, aiding in the diagnosis of challenging infections like Methicillin-Resistant Staphylococcus aureus (MRSA).

Methicillin – 5 μg Antibiotic Disc – Bioanalyse Key Features

• Standardized Potency: Each disc contains a precisely measured 5 μg of Methicillin, ensuring consistent and reliable test results.
• High-Quality Impregnation: Utilizes advanced techniques for uniform drug distribution on high-grade paper discs, promoting accurate zone of inhibition development.
• Ease of Use: Designed for straightforward application in standard laboratory workflows, simplifying the antimicrobial susceptibility testing process.
• MRSA Detection Focus: Primarily employed to screen for bacterial strains exhibiting resistance to Methicillin, a vital step in managing MRSA infections.
• Cost-Effective Screening: Offers an economical yet effective method for initial susceptibility screening in clinical and research settings.
• Trusted Brand: Manufactured by Bioanalyse, a recognized name in quality laboratory diagnostics, ensuring product integrity and performance.

Technical Specifications

• Product: Methicillin Antibiotic Disc
• Potency: 5 μg per disc
• Method of Application: Disc Diffusion Method (Kirby-Bauer)
• Intended Use: Antimicrobial Susceptibility Testing (AST)
• Target Pathogen Identification: Key for MRSA screening
• Manufacturer: Bioanalyse
• Format: Paper discs, impregnated
• Storage Recommendations: Typically refrigerated, as per manufacturer’s guidelines for optimal potency preservation.

Interpreting Results with Methicillin Discs

The interpretation of results from the Methicillin – 5 μg Antibiotic Disc is based on the diameter of the zone of inhibition surrounding the disc after incubation. Standardized guidelines are followed:

• Zone Size ≥ 28 mm: Indicates the bacterial isolate is Susceptible to Methicillin.
• Zone Size 23-27 mm: Suggests an Intermediate susceptibility, where treatment might be effective with higher dosages or specific conditions.
• Zone Size < 23 mm: Denotes that the bacterial isolate is Resistant to Methicillin.

Important Note on Interpretation: The disc diffusion method is a semi-quantitative technique. While valuable for screening, more precise quantitative methods like broth microdilution or agar dilution are recommended for definitive susceptibility profiling. Factors such as agar type, incubation temperature, and inoculum density can influence results, necessitating strict adherence to standardized laboratory protocols for accurate and reproducible outcomes.