Himedia Mueller Hinton Agar 500gm

HiMedia Mueller Hinton Agar 500gm

HiMedia Mueller Hinton Agar (MHA) 500gm is a high-quality dehydrated microbiological culture media used primarily for Antimicrobial Susceptibility Testing (AST). It is the recommended standard medium for the Kirby-Bauer disk diffusion method as per CLSI guidelines. This medium supports the growth of a wide range of non-fastidious bacteria and is widely used in clinical, diagnostic, pharmaceutical, and research laboratories.

Manufactured by HiMedia Laboratories, this product ensures reliable, reproducible, and internationally accepted results for antibiotic sensitivity testing.

Intended Use

Recommended for the determination of susceptibility of microorganisms to antimicrobial agents isolated from clinical specimens.

Key Features

• Pack Size: 500gm

• Dehydrated powder culture media

• Standard medium for AST (Kirby-Bauer method)

• pH: 7.3 ± 0.1 at 25°C

• Excellent batch-to-batch consistency

• Made in India

• Suitable for clinical & research laboratories

General Composition & Function of Ingredients

• Beef Infusion: Provides nitrogen, vitamins, and essential nutrients.

• Casein Hydrolysate (Acicase): Supplies amino acids and peptides for bacterial growth.

• Starch: Absorbs toxic metabolites that may interfere with antibiotic activity.

• Agar: Solidifying agent for plate preparation.

• Distilled Water: Used for dissolving the medium components.

Applications

 Antibiotic susceptibility testing (AST)
 Kirby-Bauer disk diffusion method
 Routine bacterial culture of non-fastidious organisms
Clinical microbiology laboratories
 Pharmaceutical quality control testing

Advantages

• Globally accepted and standardized medium

• Ensures consistent and reproducible antibiotic diffusion

• Controlled calcium and magnesium levels for accurate AST results

• Supports growth of most common bacterial pathogens

Precautions

• Not recommended for fastidious organisms such as Neisseria or Haemophilus species.

• Agar depth should be maintained at 4 mm for accurate zone of inhibition measurement.

• Prepare and sterilize as per recommended laboratory protocols.

HiMedia Mueller Hinton Agar 500gm is an essential culture medium for determining bacterial resistance and guiding effective antibiotic therapy in clinical settings.

https://www.himedialabs.com/media/TD/M173.pdf